{
  "id": "skill_1e197cad364d44be84b0e57f70fb471f",
  "name": "sgrna-design",
  "description": "Find or design sgRNAs (guide RNAs) for CRISPR experiments using a three-tiered approach: (1) validated sequences from a bundled Addgene database + literature search, (2) precomputed CRISPick designs from the Broad Institute GPP, (3) de-novo design rules. Use whenever someone asks for a guide RNA / sgRNA / CRISPR guide to knock out (KO), activate (CRISPRa), or inhibit (CRISPRi) a gene, mentions SpCas9 / SaCas9 / AsCas12a / enAsCas12a, asks \"what guide should I use for <gene>\", or wants CRISPR targeting sequences for a screen — even if they don't say \"design\". Covers human, mouse, rat, and other model organisms. Does NOT run wet-lab validation or genome-wide off-target alignment.",
  "category": "general",
  "owner_type": "user",
  "created_by_name": "Yuanhao Qu",
  "verified": false,
  "icon": "flask",
  "keywords": [],
  "default_message": "",
  "created_at": "2026-06-03T02:58:10.953000",
  "updated_at": "2026-06-03T03:41:55.161000",
  "entrypoint": "SKILL.md",
  "supporting_files": {
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    "references/design_rules.md": {
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    "references/refresh_resources.md": {
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    "references/resource/CRISPick_download_links.txt": {
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    "references/resource/addgene_grna_sequences.csv": {
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    "scripts/__init__.py": {
      "type": "py",
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    "scripts/check_design_rules.py": {
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    "scripts/export_results.py": {
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    "scripts/find_crispick_dataset.py": {
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    "scripts/search_addgene.py": {
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    },
    "scripts/select_crispick_sgrnas.py": {
      "type": "py",
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  },
  "_category_display": "General"
}